SUMMARY THIAB EXPLANATION THEM NYIAJ
Malaria yog kab mob yoov tshaj cum, hemolytic, febrile uas kis tau ntau tshaj 200 lab tus tib neeg thiab tua ntau dua 1 lab tus tib neeg hauv ib xyoos.Nws tshwm sim los ntawm plaub hom Plasmodium: P. falciparum, P. vivax, P. ovale, thiab P. malariae.Cov plasmodia no kis tau thiab rhuav tshem tib neeg erythrocytes, ua kom txias, ua npaws, anemia, thiab splenomegaly.P. falciparum ua rau muaj kab mob ntau dua li lwm hom plasmodial thiab suav rau feem ntau cov neeg mob malaria tuag.P. falciparum thiab P. vivax yog cov kab mob ntau tshaj plaws, txawm li cas los xij, muaj ntau qhov sib txawv ntawm thaj chaw hauv hom kev faib tawm.
Kev lig kev cai, malaria raug kuaj pom los ntawm kev ua qauv qhia ntawm cov kab mob ntawm Giemsa stained tuab smears ntawm peripheral ntshav, thiab ntau hom plasmodium yog qhov txawv los ntawm lawv cov tsos mob erythrocytes1.Cov txheej txheem muaj peev xwm kuaj xyuas qhov tseeb thiab txhim khu kev qha, tab sis tsuas yog thaum ua los ntawm cov kws tshaj lij cov kws kho mob siv cov txheej txheem 2, uas nthuav tawm cov teeb meem loj rau thaj chaw deb thiab tsis zoo hauv ntiaj teb.
Malaria Pf / Pan Antigen Rapid Test Kit yog tsim los daws cov teeb meem no.Qhov kev sim siv ib khub ntawm monoclonal thiab polyclonal antibodies rau P. falciparum cov protein tshwj xeeb, Histidine Repeat Protein II (pHRP-II), thiab ib khub ntawm monoclonal antibodies rau plasmodium Lactate Dehydrogenase (pLDH), ib qho protein uas tsim los ntawm plaub hom ntawm cov plasmodium, yog li ua kom pom ib txhij thiab sib txawv ntawm tus kab mob P. falciparum thiab los yog ib qho ntawm peb plasmodia.Nws tuaj yeem ua tau los ntawm cov neeg ua haujlwm tsis muaj kev cob qhia lossis cov neeg ua haujlwm tsawg, tsis muaj cov cuab yeej kuaj.
PRINCIPLE
Pf/ Pan Malaria Rapid Test kit yog ib qho lateral flow chromatographic immunoassay.Cov khoom siv tshuaj ntsuam xyuas muaj xws li: 1) burgundy coloured conjugate ncoo uas muaj nas anti- pHRP-II antibody conjugated nrog colloid kub (pHRP II-kub conjugates) thiab nas anti-pLDH antibody conjugated nrog colloid kub (pLDH-kub conjugates),
2) nitrocellulose membrane strips uas muaj ob lub xeem bands (yias thiab Pv bands) thiab ib tug tswj band (C band).Pan band yog pre-coated nrog monoclonal anti-pLDH cov tshuaj tiv thaiv los ntawm kev kis tus kab mob nrog ib qho ntawm plaub hom plasmodia tuaj yeem kuaj pom, Pf band yog pre-coated nrog polyclonal anti-pHRP-II cov tshuaj tiv thaiv rau kev kuaj pom tus kab mob Pf, thiab C band yog coated nrog tshis tiv thaiv nas IgG.
Thaum lub sij hawm ntsuam xyuas, ib qho txaus ntim ntawm cov ntshav kuaj tau muab xa mus rau hauv cov qauv zoo (S) ntawm qhov ntsuas cassette, ib qho lysis tsis yog ntxiv rau qhov tsis zoo (B).Cov tsis muaj cov tshuaj ntxuav tes uas lyses cov qe ntshav liab thiab tso tawm ntau yam plasmodium antigens, uas tsiv los ntawm capillary kev txiav txim siab hla cov sawb tuav hauv lub cassette.pHRP-II yog muaj nyob rau hauv cov qauv yuav khi rau pHRP II-kub conjugates.Lub immunocomplex yog ces ntes tau ntawm daim nyias nyias los ntawm pre-coated anti-pHRPII cov tshuaj tiv thaiv, tsim ib tug burgundy xim Pf band, qhia tau hais tias ib tug Pf zoo xeem.pLDH yog muaj nyob rau hauv cov qauv yuav khi rau pLDH kub conjugates.Lub immunocomplex yog ces ntes tau ntawm daim nyias nyias los ntawm pre-coated anti pLDH antibody, tsim ib tug burgundy xim Pan band, qhia tau hais tias ib tug plasmodium zoo xeem.Thaum tsis muaj Pan band, qhov kev kuaj pom zoo rau ib qho ntawm peb qhov plasmodia tuaj yeem pom zoo.
Tsis muaj ib qho kev xeem qhab nia (Yias thiab Pf) qhia tias qhov tshwm sim tsis zoo.Qhov kev sim no muaj kev tswj hwm sab hauv (C band) uas yuav tsum tau nthuav tawm cov xim burgundy ntawm cov tshuaj tiv thaiv kab mob ntawm tshis tiv thaiv nas IgG / nas IgG (pHRP-II thiab pLDH-kub conjugates) tsis hais cov xim kev loj hlob ntawm ib qho ntawm cov ntawv xeem. .Txwv tsis pub, cov txiaj ntsig kev xeem tsis raug cai thiab cov qauv yuav tsum tau rov kuaj dua nrog lwm lub cuab yeej.
