SUMMARY THIAB EXPLANATION THEM NYIAJ
Tuberculosis yog ib yam kab mob sib kis tau los ntawm M. TB hominis (Koch's bacillus), qee zaus los ntawm M. TB bovis.Lub ntsws yog lub hom phiaj tseem ceeb, tab sis txhua yam hauv nruab nrog cev yuav kis tau.
Qhov kev pheej hmoo ntawm tus kab mob TB tau poob qis zuj zus nyob rau xyoo pua 20th.Txawm li cas los xij, qhov tshwm sim tsis ntev los no ntawm cov tshuaj tiv thaiv kab mob 1, tshwj xeeb tshaj yog cov neeg mob AIDS2, tau ua rau muaj kev txaus siab rau TB.Qhov xwm txheej kis tau tshwm sim nyob ib puag ncig 8 lab tus neeg mob hauv ib xyoos nrog kev tuag ntawm 3 lab ib xyoos.Kev tuag ntau dua 50% hauv qee lub tebchaws African uas muaj tus kabmob HIV siab.
Thawj qhov kev xav tsis thoob thiab cov kev tshawb pom radiographic, nrog rau tom qab kuaj pom los ntawm kev kuaj hnoos qeev thiab kab lis kev cai yog cov txheej txheem ib txwm siv hauv kev kuaj mob TB5,6.Txawm li cas los xij, cov txheej txheem no tsis muaj kev nkag siab lossis siv sijhawm, tshwj xeeb yog tsis haum rau cov neeg mob uas tsis tuaj yeem tsim cov hnoos qeev txaus, smear-negative, lossis xav tias muaj cov kab mob ntsws ntxiv.
TB IgG/IgM Combo Rapid Test yog tsim los daws cov teeb meem no.Kev kuaj pom IgM thiab IgG tiv thaiv MTB hauv cov ntshav, ntshav, lossis ntshav tag nrho hauv 15 feeb.Ib qho txiaj ntsig zoo IgM qhia tau tias muaj tus kab mob MTB tshiab, thaum qhov lus teb zoo ntawm IgG qhia tau tias muaj kab mob yav dhau los lossis mob ntev.Siv M.TB cov tshuaj tiv thaiv tshwj xeeb, nws kuj kuaj pom IgM los tiv thaiv MTB hauv cov neeg mob uas tau txhaj tshuaj BCG.Tsis tas li ntawd, qhov kev xeem tuaj yeem ua tau
ua los ntawm cov neeg tsis muaj kev cob qhia lossis cov neeg ua haujlwm tsawg tsawg uas tsis muaj cov cuab yeej kuaj sim.
PRINCIPLE
TB IgG/IgM Rapid Test yog ib qho lateral flow chromatographic immunoassay.Cov ntawv xeem muaj xws li: 1) ib lub burgundy coloured conjugate ncoo uas muaj M.TB antigens conjugated nrog colloid kub (MTB conjugates) thiab luav IgG-kub conjugates, 2) ib tug nitrocellulose membrane strips uas muaj ob lub xeem bands (M thiab G bands. ) thiab tswj band (C band).M band yog pre-coated nrog monoclonal anti-tib neeg IgM rau kev tshawb nrhiav ntawm IgM anti-MTB, G band yog pre-coated nrog reagents rau kev tshawb pom ntawm IgG anti-MTB, thiab C band yog ua ntej. -coated nrog tshis tiv thaiv luav IgG.
Thaum lub ntim ntim txaus ntawm cov qauv kuaj tau muab xa mus rau hauv cov qauv zoo ntawm lub cassette, cov qauv txav mus los ntawm kev ua haujlwm capillary hla lub cassette.IgM anti-M.TB yog muaj nyob rau hauv cov qauv yuav khi rau MTB conjugates.Tom qab ntawd cov immunocomplex raug ntes ntawm daim nyias nyias los ntawm cov txheej txheem ua ntej-coated los tiv thaiv tib neeg IgM cov tshuaj tiv thaiv, tsim cov xim burgundy M band, qhia txog MTB IgM qhov kev kuaj pom zoo.IgG anti- M.TB, yog muaj nyob rau hauv cov qauv, yuav khi rau MTB conjugates.Lub immunocomplex yog tom qab ntawd ntes los ntawm pre-coated reagents ntawm daim nyias nyias, tsim burgundy xim G band, qhia txog MTB IgG qhov kev kuaj pom zoo.Tsis muaj ib qho kev xeem qhab nia (M thiab G) qhia tias qhov tshwm sim tsis zoo.Qhov kev sim no muaj kev tswj hwm sab hauv (C band) uas yuav tsum tau nthuav tawm cov xim burgundy ntawm cov tshuaj tiv thaiv kab mob ntawm tshis anti luav IgG / luav IgG-kub conjugate tsis hais txog xim kev loj hlob ntawm ib qho ntawm T bands.Txwv tsis pub, cov txiaj ntsig kev xeem tsis raug cai thiab cov qauv yuav tsum tau rov kuaj dua nrog lwm lub cuab yeej.
